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666 781 cterm  (Addgene inc)


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    Structured Review

    Addgene inc 666 781 cterm
    666 781 Cterm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/666+781+cterm/pcDNA3%2E1_hE2F7mg_PP7-tag+(Plasmid+%2373078)/bio_rxiv__2022__08__30__505852-28-27-12
    Average 93 stars, based on 2 article reviews
    666 781 cterm - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: β-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription
    Article Snippet: .. A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM), 666-750, 666-781 (CTERM), 730-781 (TAD2), and 750-781) were amplified by PCR and cloned into a pET21b vector using Eco RI, Bam HI and Xho I restriction enzymes, downstream of sequences encoding for a hexahistidine tag, the B1 domain of Streptococcus protein G (GB1), and a tobacco etch virus (TEV) protease cleavage site to create pGB1-βCAT plasmids. ..

    Amplification:

    Article Title: β-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription
    Article Snippet: .. A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM), 666-750, 666-781 (CTERM), 730-781 (TAD2), and 750-781) were amplified by PCR and cloned into a pET21b vector using Eco RI, Bam HI and Xho I restriction enzymes, downstream of sequences encoding for a hexahistidine tag, the B1 domain of Streptococcus protein G (GB1), and a tobacco etch virus (TEV) protease cleavage site to create pGB1-βCAT plasmids. ..

    Polymerase Chain Reaction:

    Article Title: β-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription
    Article Snippet: .. A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM), 666-750, 666-781 (CTERM), 730-781 (TAD2), and 750-781) were amplified by PCR and cloned into a pET21b vector using Eco RI, Bam HI and Xho I restriction enzymes, downstream of sequences encoding for a hexahistidine tag, the B1 domain of Streptococcus protein G (GB1), and a tobacco etch virus (TEV) protease cleavage site to create pGB1-βCAT plasmids. ..

    Clone Assay:

    Article Title: β-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription
    Article Snippet: .. A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM), 666-750, 666-781 (CTERM), 730-781 (TAD2), and 750-781) were amplified by PCR and cloned into a pET21b vector using Eco RI, Bam HI and Xho I restriction enzymes, downstream of sequences encoding for a hexahistidine tag, the B1 domain of Streptococcus protein G (GB1), and a tobacco etch virus (TEV) protease cleavage site to create pGB1-βCAT plasmids. ..

    Virus:

    Article Title: β-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription
    Article Snippet: .. A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM), 666-750, 666-781 (CTERM), 730-781 (TAD2), and 750-781) were amplified by PCR and cloned into a pET21b vector using Eco RI, Bam HI and Xho I restriction enzymes, downstream of sequences encoding for a hexahistidine tag, the B1 domain of Streptococcus protein G (GB1), and a tobacco etch virus (TEV) protease cleavage site to create pGB1-βCAT plasmids. ..



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    93
    Addgene inc 666 781 cterm
    666 781 Cterm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/666+781+cterm/pcDNA3%2E1_hE2F7mg_PP7-tag+(Plasmid+%2373078)/bio_rxiv__2022__08__30__505852-28-27-12
    Average 93 stars, based on 1 article reviews
    666 781 cterm - by Bioz Stars, 2026-10
    93/100 stars
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